BI478基因工程（B类）课程教学大纲

基因工程(B类)课程教学大纲课程基本信息(Course Information)课程代码*学时*学分B1478322(Course Code)(Credit Hours)(Credits)*课程名称(中文)基因工程(B类)Course Name(英文)Genetic Engineering(B)课程性质培养计划内课程(Course Type)授课对象生物工程专业本科生(Audience)授课语言中文(Language of Instruction)*开课院系生命科学技术学院(School)先修课程生物学导论，分子生物学，微生物学(Prerequisite)授课教师白林泉课程网址(Instructor)(Course Webpage)《基因工程》是生物工程一级学科的核心课程和现代生物技术产业的主要支撑，既是分子生物学、遗传学、微生物学等基础生物学向应用的延伸，也是生物技术产业发展的理论和技术基础。本课程主要包括三个部分：一是分子生物学基础，主要包括复制、转录和翻译过程：二是分子克隆技术，主要包括基因的获得、DNA重组、重组分子转移、克隆的筛选、目标产物高产与优产等环节：三是基因*课程简介(Description)工程技术在不同物种中的应用，主要包括大肠杆菌、酵母菌、放线菌、真菌、昆虫、动物等。课程教学目标包括以下三个方面：一是巩固基因工程的相关理论基础，做到理论解释和指导实验、活学活用：二是掌握分子克隆的基本技术及其原理，了解基因工程的设计与技术集成理念：三是了解基因工程在生物技术产业中的巨大贡献与潜力，激发专业学习热情与远大抱负。Genetic Engineering is one of the corner stones for Bioengineering andBiotechnology discipline and the modern biotechnology industry.It is the extensionof Molecular Biology,Genetics,and Microbiology toward application.Also,it servesas the theoretical and applied basis for the development of biotechnology.The maincontents include three parts.The first part is the related theories of Molecular*课程简介(Description)Biology,mainly including DNA replication,transcription,and translation processes.The second part are core experimental techniques,such as isolation of target genes,DNA recombination,transfer of the recombined DNA,screening of clones,andoptimized production of target molecules.The third part is a few applicationexamples of genetic engineering in different organisms,including E.coli,yeast,actinobacteria,fungi,insect,and animal.This course aims to deliver related theories