thermo-dionex-ultimate-3000_instrumental-操作说明书手册

Instrumental operation:Ultimate 3000Standard Operational ProcedureJaggerInstrumental operation:Ultimate 3000ScopeThe scope of this document is to present an overview of the high performance liquid chromatography(HPLC)system Ultimate 3000 and to provide the reader with guidelines for standard operations,routinemaintenance and simple trouble shooting.Detailed instrument description can be found in DionexReference Library (CD-ROM)which contains all relevant and specific manuals.Instrument overviewThe Dionex Ultimate 3000 system is a dual pump system with three channels on each pump.It is capableof operating two columns simultaneously via the "LEFT"pump and the "RIGHT"pump.In practice thecommon use will be one column at a time,and therefore regular changes between the two pumps willbe necessary to prolong the lifetime of the system.The detection principle is based on UV-VIS(ultraviolet-visible light)detection and up to four different wavelengths can be detected and logged atthe same time;ranging from 190 to 740 nm.Figure 1 shows an instrument overview.HPLC systems are delicate equipment and should be handled accordingly.Regularly check the status ofthe instrument,preferably on a daily basis and always be careful when operating.Eluents shouldALWAYS be prepared from fresh top quality water (Milli-Q/18.2 MO-cm)and preferably samplepreparation should also be made based on this water quality.The chromatographic system is controlled by a computer installed with ChromeleonTM 7 software(hereafter CM)version 7.1.1.1127 for instrument control and data handling.All commands andinstrument handlings goes through this software.Page 1 of 21Edition 01