JISL1902-2015

L1902:20153.5 antibacterial efficacyefficacy of antibacterial activity shown by antibacterial finishIt is evaluated by the existence of halo and antibacterial activity value.3.6 plate count methodmethod in which the number of bacteria present after incubation is calculated by count-ing the number of colonies according to a ten-time dilution methodThe results are expressed in"CFU (Colony Forming Unit)"3.7 luminescence methodmethod in which the amount of adenosine triphosphate (hereafter referred to as "ATP")contained in bacterial cells is measuredThe results are expressed in "moles of ATP".3.8 neutralizerchemical agents used to inactivate,neutralize or quench the antibacterial properties ofantibacterial agents3.9 halothe part where the growth of test bacteria produced around the sample is suppressedwhen the antibacterial processed sample is placed and incubated on the culture mediumcontaining the test bacteria4 Safety preeautionThe test methods specified herein require the use of bacteria.These tests should becarried out by person with training and experience in the use of microbiological tech-niques.Safety precautions shall be checked against and comply with regulations.5 Apparatus5.1 Spectrophotometer,capable of measuring at a 620 nm to 660 nm wavelength,orMeFarland's nephelometer.5.2 Ineubator,capable of maintaining a constant temperature of 37'C+2C.5.3 Water baths,one capable of maintaining a constant temperature of 46Ct2Cand another capable of maintaining a temperature of 70C to 90C.5.4 Mixer,producing a vortex shaking action.5.5 Stomacher,capable of speeds of 6 blows per second to 8 blows per second,withthe corresponding disposable containers.5.6 Clean bench or safety cabinet,for microbial test.Safety cabinet is specified inJIS K 38005.7 Platinum colony loop,with a loop of approximately 4 mm in diameter5.8 Humidity chamber,tropical chamber or other container capable of maintaininga high-humidity more than 70%RH atmospheric condition.